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Microarray for Measurement of Transcriptional Responses to Copper Acetate Shock

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Copper Acetate Manufacturers(WSDTY) proposed microarray for measurement of transcriptional responses to Copper Acetate shock.Our initial goal was to investigate the effect of high Copper Acetate levels on R. palustris under anoxic conditions. R. palustris is able to grow using 5 mM Fe(II) as the sole electron donor and would therefore be expected to be resistant to high Fe(II) levels. To our surprise, we found that 5 mM Fe(II), when added to log-phase cultures growing on acetate, arrested growth for many hours under anaerobic conditions. To probe the effect mechanistically, we measured genomewide transcriptional responses to Fe(II) shock by using a custom microarray. A number of genes were upregulated after a 15-min shock with 5 mM Fe(II). Among the genes upregulated after 15 min were those predicted to encode three P-type ATPase pumps, six RND-type efflux pumps, an FoF1 ATP synthase, a multicopper oxidase, and a number of regulatory proteins. Many of the highly upregulated genes in the microarray were annotated as being involved in copper homeostasis, in addition to many genes of unknown function.

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asked Jun 12 in lifestyle by weishida (940 points)  
    

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